nonlinear regression curve fit with a variable slope and graphpad prism 4.0 software Search Results


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Xenova Group xr9576
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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LI-COR odyssey imaging system
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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LI-COR odyssey
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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OriginLab corp originpro7 software
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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Bio-Techne corporation a 485
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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Sartorius AG octet
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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Sartorius AG octet bli systems
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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Becton Dickinson carv ii tm confocal imager
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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NanoTemper Technologies monolith nt.115
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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LI-COR irdye 800cw nhs
FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor <t>XR9576</t> on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.
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Image Search Results


FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor XR9576 on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.

Journal: Journal of Biological Chemistry

Article Title: Inhibition of Glucosylceramide Synthase Does Not Reverse Drug Resistance in Cancer Cells

doi: 10.1074/jbc.m404466200

Figure Lengend Snippet: FIG. 1. Representative example of a cytotoxicity assay. Results are for the effect of the P-glycoprotein inhibitor XR9576 on the cytotox- icity of doxorubicin in NCI/AdRRES cells. Cytotoxicity assays were car- ried out as described under “Experimental Procedures.” Results are represented as (E, thin solid line) MCF7-wt untreated; (G, thick solid line) NCI/AdRRES untreated; ( , dashed line) NCI/AdRRES 25 nM XR9576. Results for MCF7-wt cells treated with 25 nM XR9576 were superimposable with those of untreated MCF7-wt cells (data not shown). Data represent the mean S.E. of at least four independent experiments.

Article Snippet: The FACSCalibur instrument was purchased with a grant from The Wellcome Trust (UK), Prism 4.0 was a kind gift from GraphPad, and XR9576 was a kind gift from Xenova Ltd.

Techniques: Cytotoxicity Assay